Serological survey to detect antigens that cross-react with COVID-19 antibodies

Main Article Content

Ahmed N. Mnahi
Abdulelah A. Almyah
Zuhair AL-Shaheen

Keywords

COVID-19, ELISA , bacterial antigens, vaccinated and unvaccinated individuals

Abstract

Background: COVID-19 first emerged in Wuhan City and has since spread worldwide, infecting over 600 million people. The World Health Organization (WHO) issued an emergency in public health in January 2020 due to reports of new coronaviruses in Europe, Asia, and the Americas. Aim: The study aims to screen bacterial antigens for cross-reactivity with COVID-19 antibodies using indirect ELISA and to detect cross-reactivity between vaccinated and unvaccinated healthy individuals and various bacterial types (Gammaproteobacteria, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, and Enterobacteria, in addition to Staphylococcus aureus) either sonicated or non-sonicated. Methods: ELISA is a method for detecting unknown samples in a sample. It involves pipetting 50 μl of diluted antigen dilution into PVC microtiter plates, with pure antigen test samples pipetted at 2 µg/ml. The plate is incubated at room temperature for 2 hours or overnight at 4°C. Blocking is done with 200 μl of blocking buffer. The plate is then incubated overnight at 4°C or room temperature for 2 hours. Primary and secondary antibody incubation is performed, with two hours of room-temperature incubation and overnight incubation for stronger staining. Absorbance is measured for each well, and the results can be analyzed using a standard curve. Results: Results showed that vaccinated individuals had highly significant p-values of IgG antibodies against S. aureus, Pseudomonas aeruginosa, Enterobacteria, and Bacillus, while unvaccinated individuals had non-significant p values. Conclusions: The high cross-reactivity identified E. coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Enterobacteria, and Gammaproteobacteria. The current study was validated by the discovery of a significant correlation between Pfizer vaccinated individual's serum antibodies and unvaccinated individual's serum and the examined bacterial antigens that had a high titer of cross-reactivity with the vaccinated samples in comparison to the unvaccinated samples (less titer).

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References

1. Al Hajjar S, Memish ZA, Mcintosh K. Middle East respiratory syndrome coronavirus (MERS-CoV): a perpetual challenge. Ann Saudi Med. 2013 Oct;33(5):427–36. https://doi.org/10.5144/0256-4947.2013.427
2. Li Q, Guan X, Wu P, Wang X, Zhou L, Tong Y, et al. Early transmission dynamics in Wuhan, China, of novel coronavirus–infected pneumonia. N Engl J Med. 2020; DOI: 10.1056/NEJMoa2001316
3. Guan W, Ni Z, Hu Y, Liang W, Ou C, He J, et al. Clinical characteristics of coronavirus disease 2019 in China. N Engl J Med. 2020;382(18):1708–20. DOI: 10.1056/NEJMoa2002032
4. Yousif II, Hashim AR, Farid HA. The clinical manifestation of post COVID-19 syndrome among Basra city population-southern of Iraq. Ann Rom Soc Cell Biol. 2021;5867–73.
5. WHO. WHO Coronavirus (COVID-19) Dashboard | WHO Coronavirus (COVID-19) Dashboard with Vaccination Data [14 April 2023]. Available from: https://covid19.who.int/
6. Rabaan AA, Al-Ahmed SH, Haque S, Sah R, Tiwari R, Malik YS, et al. SARS-CoV-2, SARS-CoV, and MERS-COV: a comparative overview. Infez Med. 2020;28(2):174–84.
7. Onofrio L, Caraglia M, Facchini G, Margherita V, Placido S De, Buonerba C. Toll-like receptors and COVID-19: a two-faced story with an exciting ending. Future Sci OA. 2020 Published online: 30July2020:FSO605. https://doi.org/10.2144/fsoa-2020-0091
8. Wrapp D, Wang N, Corbett KS, Goldsmith JA, Hsieh C-L, Abiona O, et al. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science 2020;367(6483):1260–3. DOI: 10.1126/science.abb2507
9. Cevik M, Bamford CGG, Ho A. COVID-19 pandemic—a focused review for clinicians. Clin Microbiol Infect. 2020;26(7):842–7. https://doi.org/10.1016/j.cmi.2020.04.023
10. Sanyaolu A, Okorie C, Marinkovic A, Patidar R, Younis K, Desai P, et al. Comorbidity and its impact on patients with COVID-19. SN Compr Clin Med. 2020;2(8):1069–76.
11. McArthur L, Sakthivel D, Ataide R, Chan F, Richards JS, Narh CA. Review of burden, clinical definitions, and management of COVID-19 cases. Am J Trop Med Hyg. 2020;103(2):625. doi: 10.4269/ajtmh.20-0564
12. Dhand R, Li J. Coughs and sneezes: their role in transmission of respiratory viral infections, including SARS-CoV-2. Am J Respir Crit Care Med. 2020;202(5):651–9.
13. Stadnytskyi V, Anfinrud P, Bax A. Breathing, speaking, coughing or sneezing: what drives transmission of SARS‐CoV‐2? J Intern Med. 2021;290(5):1010–27.
14. Jarrom D, Elston L, Washington J, Prettyjohns M, Cann K, Myles S, et al. Effectiveness of tests to detect the presence of SARS-CoV-2 virus, and antibodies to SARS-CoV-2, to inform COVID-19 diagnosis: a rapid systematic review. BMJ Evidence-Based Med. 2022;27(1):33–45. http://dx.doi.org/10.1136/bmjebm-2020-111511
15. Gan SD, Patel KR. Enzyme immunoassay and enzyme-linked immunosorbent assay. J Invest Dermatol. 2013;133(9):e12. https://doi.org/10.1038/jid.2013.287
16. Hosseini S, Vázquez-Villegas P, Rito-Palomares M, Martinez-Chapa SO. Enzyme-linked immunosorbent assay (ELISA): from A to Z. Springer. 2018. book series (MIMB,volume 32). https://doi.org/10.1007/978-981-10-6766-2
17. Clark BR, Engvall E. Enzyme-linked immunosorbent assay (ELISA): theoretical and practical aspects. in: Enzyme-immunoassay. CRC Press; 2018 p. 167–80. eBook ISBN: 9781351071840 https://doi.org/10.1201/9781351071840
18. Rabaan AA, Al-Ahmed SH, Sah R, Tiwari R, Yatoo M, Patel SK, et al. SARS-CoV-2/COVID-19 and advances in developing potential therapeutics and vaccines to counter this emerging pandemic. Ann Clin Microbiol Antimicrob. 2020;19(1):1–37.
19. Uttarilli A, Amalakanti S, Kommoju P-R, Sharma S, Goyal P, Manjunath GK, et al. Super-rapid race for saving lives by developing COVID-19 vaccines. J Integr Bioinform. 2021;18(1):27–43.
20. Sell S. How vaccines work: immune effector mechanisms and designer vaccines. Expert Rev Vaccines. 2019;18(10):993–1015.
21. Ellis H. James Phipps, first to be vaccinated against smallpox by Edward Jenner. J Perioper Pract. 2021;31(1–2):51–2.
22. Almayah AA. ELISA for screening and diagnosis of infected and non-infected people by epidemic and non-epidemic V. cholerae. Mesopotamian J Mar Sci. 2009;24(Pages 148-159).
23. Li Z, Yi Y, Luo X, Xiong N, Liu Y, Li S, et al. Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis. J Med Virol. 2020;92(9):1518–24.
24. Mahajan S, Rajput TA, Sharma N, Duara P. COVID 19: Impact and response (volume III).
25. Yang L, Liu S, Liu J, Zhang Z, Wan X, Huang B, et al. COVID-19: immunopathogenesis and immunotherapeutics. Signal Transduct Target Ther. 2020;5(1):128.
26. Root-Bernstein R. COVID-19 coagulopathies: Human blood proteins mimic SARS-CoV-2 virus, vaccine proteins and bacterial co-infections inducing autoimmunity: combinations of bacteria and SARS-CoV-2 synergize to induce autoantibodies targeting cardiolipin, cardiolipin-binding. BioEssays. 2021;43(12):11–3.
27. Prompetchara E, Ketloy C, Palaga T. Immune responses in COVID-19 and potential vaccines: lessons learned from SARS and MERS epidemic. Asian Pacific J allergy Immunol. 2020;38(1):1–9.

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